Veterinary Microbiology Laboratory Manual Pdf
Watch from the side so that you avoid damaging the lens by running it into the slide. Please complete the required fields. Flagella can also be demonstrated by the light microscope, using special staining methods which require most careful attention to detail technique. Many fungal infections are similar clinically to mycobacterial infections, and often the same clinical specimen is cultured for both fungi and mycobacteria.
Irradiation in which rays such as ultraviolet, gamma rays and x-rays damage microorganisms affecting the cell membranes, essential enzymes or the nucleic acids. The number of choices is excessive and the consumer is often unaware that many of the anti-microbial agents are no more effective than basic soap and water.
Demonstration of major laboratory equipment and reagents Microscopes Microscopes are one of the most important tools available to a microbiologist. The main portion of this manual involves the identification of unknown microbial agents. Easy to adapt for almost any microbiology lab course, this versatile, comprehensive, and clearly written manual is competitively priced and can be paired with any undergraduate microbiology text.
Such an assessment will take the risk group as well as other containment factors into consideration in establishing the appropriate biosafety level. The factor of agar dilution must be allowed for in the first calculation as follows. Hyphal growth occurs by apical elongation.
Home Microbiology Clinical Veterinary Microbiology. In this practical laboratory manual for the veterinary microbiologist, the authors provide concise information on the materials and methods of bacteriology, the secret language of competitive intelligence pdf mycology and virology.
Endospore Staining Schaeffer-Fulton or Wirtz-Conklin The endospore stain is a differential stain which stains bacterial endospores or spores. Used for sterilization of flasks, test tubes and pipettes placed in pipette canisters. Pour plates allow micro-organisms to grow both on the surface and within the medium. Chemicals shall be correctly stored in appropriate containers and at the correct temperature.
Gloves should be worn that provide insulation from cold and that are also waterproof, to prevent penetration of the liquid nitrogen. They can only be achieved by careful study of the principles involved followed by practical application to premises, facilities, operating procedures and hygiene. The Agar dilution Method Agar dilutions are most often prepared in petri dishes and have advantage that it is possible to test several organisms on each plate. Replace the lid of the Petri dish and discard the pipette in a discard jar.
Microbiology A Laboratory Manual 11th Global Edition
Transfer one or two loopfuls of tap water on to the centre of the slide. Use the needle to inoculate a nutrient agar deep tube by stabbing the needle into the agar deep. Preparation of antibiotic stock solutions Antibiotics may be received as powders or tablets. Choice, preparation and use of antiseptics and disinfectants Specific disinfectants at specified working strengths are used for specific purposes.
It is usually achieved by the use of chemicals. Hold there until it is red hot. In those cases, the endospores will appear as clear ovals or circles within a pink vegetative cell. The total volume in each tube is the same. Inoculation of media in screw cap bottles and test tubes is usually done by using a wire loop.
The primary stain in the endospore staining procedure is malachite green, which stains both vegetative cells and endospores. If the broth culture used has grown poorly, it may be necessary to use this undiluted. Plates should be used within seven days after preparation unless adequate precautions, such as wrapping in plastic, have been taken to minimize drying of the agar.
Serum samples can be examined either native or are taken in a special tubes in the deep-freeze at oC. But, once stained, they strongly resist decolonization. Make sure all caps are loose, but secure.
Veterinary eBooks PDF
Various media types used in microbiology labs include agar slants, agar deeps, agar plates, and broths. Generally there are three types of stain used in most microbiology laboratory.
This might cause colonies to spread into each other and risk the spillage of the contaminated liquid. This technique consists of labeling antibody with fluorescein isothiocyantate, a fluorescent compound with an affinity for proteins to form a complex, conjugate. This lens system then produces a virtual image that you see when you look through the microscope. Examples of simple stains include methylene blue, carbolfuchsin, crystal violet, and safranin.
PDF) VETERINARY MICROBIOLOGY LABORATORY MANUAL
Examine the isolate on the streak plate. As with the acid-fast stain, heat is required to penetrate the endospore coat. Draw the rest of the wire upwards slowly up into the hottest region of the flame, immediately above the light blue cone. However it takes longer time to sterilize, less penetration capacity and thus less efficient. Any discernable growth within zone of inhibition is indicative of methicillin or vancomycin resistance.
Because tests for antigen also have false- positive results, the tests can be used to complement one another. Lift the lid of the Petri dish to allow entry of spreader.
For primary culturing of unknown organism, ordinary enriched media like nutrient or blood agar is used. Effective treatment and preventive measures are available. These are typically inoculated by stabbing the media with a sterile needle. The precipitate is most heavy in the equivalence zone, when antigen and antibody are fully combined. The hydrogen gas combines with free oxygen in the chamber to produce water, thus removing all free oxygen from the chamber.
Nonspecific inhibitors can cause false positive results. Clinical preparations should not be used for reference technique.
Clinical Veterinary Microbiology
Laboratory work in veterinary microbiology lab should be carried out with a minimum of risk to the health of the staff biosafety and the environment biocontainment. List of equipment commonly used in Microbiology Laboratory and their functions.
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